U2OS cell line stably expressing Adenosine A2B Receptor and cAMP Biosensor

Description U2OS cell line stably expressing Adenosine A2B Receptor and cAMP Biosensor cAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP Nomad Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMP NOMAD Adenosine A2B Receptor Cell Line contains U2OS cells stably expressing the following constructs: Human Adenosine A2B receptor (ADORA2B) with no tag cAMP Nomad Biosensor (Red Fluorescence) cAMP NOMAD ADORA2B Receptor Cell Line allows for the assay of compounds that modulate the G-protein signaling pathway via Gs protein activation. When an agonist binds to ADORA2B, the Gs protein is activated, which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase in the red fluorescence intensity of the cAMP biosensor. Thus, the activity can be easily quantified in living cells in a dose-response manner by fluorescence emission in a microplate reader.